Charges are being brought against winner Gino D'Acampo and fellow contestant Stuart Manning after the chef apparently killed and butchered a rat to add meat to a meal.
According to the News of the World, the animal may have been a tame rat let loose on the set to add drama, rather than the wild variety.
During the series the contestants were divided into two groups, one of which was in "exile" with meagre rations.
As the celebrities became concerned that they would lack the energy to complete physical challenges, D'Acampo, 33, decided to take matters into his own hands. He said in the show's video diary room: "It's not done by choice but it's done because we need it. We need some kind of protein, we need some kind of flavour. I saw one of these rats running around. I got a knife, I got its throat, I picked it up."
The group, including 30-year-old Manning, ate the rat and enjoyed the meal.
Chief Inspector David Oshannessy, from the RSPCA in New South Wales, said it was not acceptable that an animal had been killed as part of a performance.
He said: "The allegation is that an animal was cruelly treated on the set. It was a rat that was killed. There is a code of conduct in New South Wales that dictates how animals can be used. The killing of a rat for a performance is not acceptable."
A spokeswoman for New South Wales Police said that there were currently no plans to bring charges against the show producers.