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The actor - the original Captain Kirk - will also be selling his Golden Globe nomination certificate and his Hollywood Walk Of Fame plaque as part of Star Trek creator Gene Roddenberry's auction of his own collection of personal effects and show memorabilia.
Hand-annotated scripts, costumes from the show and Roddenberry's own studio pass are among the items available at the auction at Planet Hollywood Resort and Casino in Las Vegas on June 27, which will be administered by Julien's Auctions.
Proceeds will benefit the Roddenberry Foundation, which makes grants to support children, education and the environment.
Shatner plans to donate a portion of the proceeds from his goods to the Hollywood Charity Horse Show, which helps handicapped children.
The Roddenberry collection will be "very meaningful" to Trek fans, said Shatner, who says the show's optimistic themes are why it has endured for decades.
"It suggests that 300 years from now, mankind is still around," the 79-year-old said. "It's on a note of hope that not only will we be around, but we will flourish and be out there among the stars, colonising."