The hit series starring Zachary as a computer geek-turned-secret agent received over 50 per cent of the total votes in E! Online's Save One Show campaign, followed by One Tree Hill and Heroes, as programmes that haven't yet been renewed for another season.
"We are so thankful that our fans voted for Chuck in such a huge way, though we are also a little frightened of their ability to control the internet - our fear aside, we are honoured to have the greatest fans in the history of television," Chuck creators Josh Schwartz and Chris Fedak told E!.
Chuck - which has had three successful seasons - joins the winners circle from the past decade of Save One Show campaigns. Previous winners include Angel, Terminator: The Sarah Connor Chronicles and Veronica Mars.