Nina says that Tony has 20 seconds to get Jamey out of the washroom. But from the time the camera switches off to back on again is more than 20 seconds.
Jamey's supposed to be receiving messages using her PDA. However, you can clearly see "Memo X of 3" above each of the messages, showing that they were pre-written onto the PDA.
Cameraman Alert! When Nina calls Tony at CTU after stumbling into the empty building, a technician holding a handheld camera can be seen in the right side of the screen. (This only appears in the widescreen version).
Milo: So what am I supposed to be doing?
Jamey: Why don't you help me format some files?
Milo: They didn't bring me in here at five in the morning to format files!
When Gaines pulls up Jamey's name on his computer among a list of other agents with other IP addresses, the names of the other agents are all names of 24 crew members.
Jack's opening monologue is introduced in this episode --"Right now, terrorists are plotting to assassinate a presidential candidate, my teenage daughter is missing, and people that I work with may be involved in both. I'm Federal Agent Jack Bauer. Today is going to be the longest day of my life." It will change over the course of the season as Jack's life changes.