Justice is still alive at the end of the episode, hinting at a second season.
Jinno does not actually die, but instead is transformed into a cyborg by the Empty Seven Clan in an attempt to kill Afro and take his headband.
There are more headbands than just the #1 and #2.
Justice's third arm reaches out of his back.
Afro and Ninja: And so, the never-ending battle continues...
Ninja Ninja: On and on and on and on.
Justice: (To Afro Samurai) Is that it? And I thought you loved your pappy. I thought all those souls you sent behind you mattered. But only now you see, your little anger is just a sea spray of blood in the endless ocean of this world's killing. It's got no end and no beginning, just the strong and the dead. Only cursed ones like you and I can stomach this truth.
Ninja Ninja: Oh man, it was the bomb hangin out wit you....Afro Samurai...
Jinno: It's been a long time, eh Afro Samurai?
Afro: You will only die again my Friend.
Jinno: Perhaps, or maybe we'll finally tie things up.
Afro: Alright... let's do this.
Justice: Don't let the name fool you--for Afro, there's nothing equitable about world's number-one warrior. Favoring lethally accurate revolvers over a sword, the three-armed Justice took down the former champion, Afro's father, and hasn't been bested since. With his glowing red eyes and odd-colored skin, Justice is clearly more than human, but that doesn't seem to intimidate Afro.