Near the end of the show, when Mike and Jason are pursuing the doctor with the virus, it is more than obvious that the pursuit was filmed in Canada, as the markings on the street were noticeably different.
Saunders: You're too late, gentlemen!
Mike: This guy's a nutcake.
Locke: And dangerous. If we shoot him, he'll drop it.
Mike: Then we'll shoot it.
Saunders: In ten seconds, the virus will thaw.....
Locke: The laser!
Saunders: .....And there's nothing you can do about it!
Locke: I'm switching to automatic. The computer will do it. I'll give coordinates.
Locke: Computer connect.
Mike: We've only got one shot at this. Are you sure?
Locke: I'm sure.
Locke: Laser's marked target.
Saunders: Four..... [Mike switches the laser to manual at the last moment]
Locke: What the hell? Mike, what are you doing?
Mike: [snaps on the visor and target box] Trust me.
Saunders: One! [Mike shoots the laser, exploding the X-Virus and Saunders]
Mike: Sorry, Locke. I guess I just put more faith in hardware than software.
Locke: I guess the force was with you, Mike.