Baby Plucky Duck (from Tiny Toon Adventures) makes a brief cameo appearance.
Slappy: Eh, that snake doesn't have a leg to stand on...(looks at audience) Hey, it's the dawn of time! What do you expect? New jokes?
(the snake has just been hit over the head)
Snake: (dizzy) Colonel Mustard did it in the kitchen...(cannon fires at him) or was it Miss Scarlet?
Slappy: (after the snake smacks into her frying pan) You remind me of a very young Scrappy-Doo.
Baby Plucky: (after the snake lands in a mini-golf course) Look, Daddy! A snake! (hits the snake with his club) Snakey go down the hole...
Snake: Colonel Mustard did it in the kitchen...or was it Miss Scarlett?
The snake is referencing the popular board game "Clue", a who-done-it? game where one must determine who killed with what weapon in what room.
Slappy: You remind me of a very young Scrappy Doo.
Scrappy Doo is the annoying nephew of Scooby Doo that never knew when to give up, much like the snake in this episode.