When Dr. Cassandra is initially handling the Alvino Ray Gun, she holds it backwards, but by the time she comes to use it on Batman, Robin and Batgirl she is using it the correct way!
King Tut appears as one of the 'arch-villains' of Gotham City, even though in a previous (two weeks before) episode 'I'll Be A Mummy's Uncle' King Tut returned to his life as a professor of Egyptology after a blow to his head that completely reformed him.
Although The Joker had a run-in with Alfred at the end of Season 2 Episode "The Joker's Provokers," he failed to recognize him in this episode.
In the Bat-fight, you can clearly see one of the Joker's henchman waiting to be 'thrown' out of the flying saucer!
This is the second and final time Batman and Robin fight the bad guys in the Batcave.
At the end of this episode, the preview for the next episode "The Joker's Flying Saucer", we see Joker's flying saucer but in the episode, he was just building his saucer.
In the pre-credits sequence, Robin is already in the Batcave with Alfred. However, when the show comes out of the main credits, because of the use of stock footage, Robin slides down the Batpole with Batman.
This was the only adventure where no words or animated signs was used.
When Batman is calculating the trajectory of the bullet Catwoman shot as a clue into Commissioner Gordan's office, he says the wind speed is six knots per hour. The unit of knot means nautical miles/hour. So Batman mistakenly turned it into acceleration as units of 6 miles per hour per hour.
The Trap that The Caped Crusader and The Boy Wonder were supposed to be placed in was cut from the story, but later in this segment The Joker is surprised when The Dynamic Duo and Batgirl appear at the lighthouse, when he believed them all to be dead! When it was decided to delete the trap sequence, nobody took notice of the goof in The Joker's dialogue!
The trap Joker is referring to is from near the end of Part 1 where his lethal Joker buzzer robbed them of their five senses one by one until Batgirl revived them with Batgirl antidote pills. Yet at the beginning of this episode, they must've seen the Batmobile drive off but were waiting to get Batgirl as well with the Cat Whiskers trap.
Robin is seen driving The Batmobile.
When Robin cuts the ship loose, Lady Peasoup says that it's drifting. In the following stock footage shot, it's clearly under power.
In the beginning of the episode, Alfred says "excellent, Master Robin" while Dick Grayson is out of "uniform" playing the drums.
In the kidnap scene, when Skip Parker leaves Barbra Gordon and arrives at the Hang Five he is barefoot. Yet when the Joker and his crew are carrying Skip Parker from the Hang Five to Joker's car, he is wear a pair of white sneakers (plimsolls) and is no longer barefoot. The sneakers look to be white canvas slip-on and he's not wearing socks.
In the scene were the villians are running from Batman and Robin, Joker's henchman were barefoot but when they ran into the building, they suddenly had shoes on and socks could be clearly seen also.
Bruce Wayne was restored to normal by listening Siren's "antidote note". Gordon and O'Hara do not receive such a treatment (and they couldn't possibly get it, even off-screen), but they are still completely fine and cured by the end of the episode.
In this episode, it is established that Barbara Gordon is Commissioner Gordon's only child, and she has no children. However, in "The Bat's Kow Tow," Gordon says that he has grandchildren.
It's interesting that Commissioner Gordon, Chief O'Hara, Bruce and Dick are in Barbara's apartment next door to where she is being held. Yet they do not hear the gun shots that the thugs make when demonstrating what will happen to the Commissioner if Barbara does not marry the Penguin!
This was the last cliffhanger for Batman and Robin.