President Uno: Shirley?
Numbuh 1600: Don't call me Shirley!
Reference to exchange of dialogue from the movie Airplane! when Robert Hays says "Surely you can't be serious?" and Leslie Nielsen replied: "I am serious, and don't call me Shirley!" This is the second time in the series they did something in reference to the comedy classic. The first was in Operation: H.O.L.I.D.A.Y. with this exchange:
Numbuh 1: (to Lizzie over the radio) Surely you realize you'll crash!
Lizzie: Who's Shirley?!