At the end of the episode, we finally see what has happened to Troy's pet monkey, Annie's Boobs, from Season 1's "Contemporary American Poultry". If you look closely, you can also see a small hand grab Annie's purple pen off the table at the beginning of the episode, while the Dean is telling everyone about the Puppy Parade.
This episode featured a new opening credits sequence that was special and exclusive for Halloween.
Abed's costume choice for his movie is possibly a reference to Gaius Baltar of the rebooted Battlestar Galactica, who rose up as a religious and spiritual leader of the surviving humans.
Throughout the entire episode, there is a story going on in the background about Abed and a pregnant woman, unknown to all the other characters.
In the opening scenes, he is talking to her behind Jeff. She goes into labor in the lunchroom and Abed leads her outside.
During the oil fight scene, if you look behind Shirley on the bench, you can see Abed delivering a baby in the back of a truck. Later you see the happy couple standing around with baby in hand.
Jeff's middle name is Tobias, as said by Alan Connor in the email sent to the State Bar Association.
Donald Glover revealed in an interview that there were actually eight giant cookies that were baked the night before taping for Troy to carry throughout the "Tranny Queen" scenes.
Britta is a vegetarian.
Britta used to live in New York.
Goof: right before Abed pulls out the picture of Britta, Jeff takes the shot of alcohol, but in the next scene Jeff is holding a shot glass that is still full.
Goof: In the scene where Britta plays Jeff's voice mail for Professor Slater (19:25-19:45), the phone begins the scene as a Samsung Gravity but switches to a Motorola ROKR E1.
Troy reacts with surprise to learn that Annie is Jewish. However Troy was present when Annie said that she is Jewish in Comparative Religion.
The sketch comedy troupe that Pierce goes to for help is actually from Donald Glover's (Troy) other project called Derrick Comedy.
Right before Troy and Britta start their duet, Madame Leclair tells the accompanist (a man with silver hair) to play something modern. But when Britta takes off her teapot hat and throws it, a woman with dark hair is playing the piano.
When Britta takes off her 'teapot' hat and throws it off stage, it hits the piano player, who promptly pushes it away with her left hand.
Dean Pelton reveals that the school has a watch list for physically attractive students and faculty. According to the Dean, Jeff is ranked #2 and Professor Slater is ranked #7.
This marks the third episode to feature Abed and Troy's 'La Biblioteca' rap. It first appeared in episode 2, "Spanish 101", and again in episode 9, "Debate 109".
Lyrics to Chang's Rap: I am Señor Chang, and I'm so ill! / This is a warning: I can't be killed! / All in your cabeza, without a chasa / Not another teacher with this much flava! / Chang!
It's revealed that Jeff is 33 years old.
Goof: Troy is mistaken -- Jehovah's Witnesses may, in fact, consume alcoholic beverages, provided that they do so in moderation (i.e. drunkenness, the misuse of alcohol, is considered the sin -- 1 Corinthians 6:10). Members of the Church of Jesus Christ of Latter Day Saints (Mormons), however, abstain from drinking of alcoholic and/or hot beverages. Also, Jehovah's Witnesses do not believe in celebrating religious holidays (which Troy does in this episode, as well as Dia De Los Muertos a few episodes earlier). Another tenet is to limit friendship and contact with those who are not fellow members of the church (something Troy certainly does not do on this show).
All of these things put together suggest that Troy has either been "disfellowshipped" (their version of an excommunication), or simply doesn't know enough about his family's religion to know what beliefs to adhere to.
Abed is Muslim, Troy is a follower of Jehovah's Witness, Shirley is Christian, Annie is Jewish, Jeff is agnostic, Britta is an atheist, and Pierce claims to be Buddhist but isn't.