Jake's car has an upside-down Anarchy symbol for a hood ornament.
At the end, when Daria and Jane are eating pizza, Jane is not wearing her black tights, but in the last shot she suddenly is wearing her tights.
Daria did not get Quinn and herself thrown out of the college. Quinn got Daria and herself thrown out of the college because Daria stayed with the tour guide and Quinn was with some Ferternity boys drinking beer.
After the camera angle zooms in and out, the statue and two books have moved slightly. Also, the blue book is now more closed than it was before the camera zoom.
In the 2nd Movie "Is it College Yet?" Jake tries to pressure Daria to go to Middleton
Helen: Seems like only yesterday I was partying with the best of them. I was pretty wild! Still am when I feel like it.
(Helen walks into a dorm and sees a boy working on his computer)
Helen: How refreshing to see someone doing school work.
Boy: Actually, I'm sending an erotic letter to a female inmate I met on the internet. I think she's female...
Teacher: You have to know how to take a test. Like, when you get a multiple choice question, you can usually eliminate two of the answers right off.
Brittany: Excuse me, sir? Does that work with true/false?
Geek: Hi, sir. This is something for your daughter, Daria. She wrote a paper for me.
Jake: And she made you pay in cash?
Geek: Yes, sir.
Jake: Good girl.
Teacher: So, how was the famous Middleton College?
Quinn: Daria got us thrown out.
Daria: I got us thrown out? I beg to differ, Keg Queen!
Kevin: Keg Queen?! Can I have your autograph?
Quinn: Shut up, Naked Boy!
Professor: Daria, I know it's only the first week of freshmen year, but I wonder if you'd consider transferring to the graduate school.
Daria: I'm not really sure I want to be a professional student.
Professor: But I don't want you to study. I want you to teach.
Professor: Not here, of course. On our Paris campus.
Daria: Oh! Okay.
Professor: Superb! Now I can use your dorm room to carry on affairs with some of the more beautiful undergraduates. Thank you!
Daria: How come even in my fantasies everyone's a jerk?
Kevin: Pst. Daria, what did you get for number one?
Daria: The thing about who I really am?
Daria: Try "cross-dresser."
Instructor: Today's admission standards are more rigorous than ever, which is why...
Jane: Can we get on with this? I have someplace to go. (looks at Jodie) Television counts as a place.
Daria: One more time: I am not taking a college prep course. Actually, I may just skip college and stay home. It'll save me the trouble of moving back in later.
(Jake swerves into traffic)
Helen: Watch the road!
Quinn: I think people who run over animals should get run over themselves to see how they like it.
Daria: What about unpopular animals?
Quinn: Unpopular animals don't count.
Daria: What about the stupid ones?
Jane: Thrown out of college without enrolling first. I am damn proud of you.
Alyson Court, who does the voice of the tour guide, Heather, is known by some as the voice of Jubilee in the cartoon version of X-Men. (She also voiced Mrs. Manson in "Esteemsters," and the voice of Lydia Deetz in Beetlejuice).
-Splendora - "You're Standing on My Neck" (closing credits)
-Maxwell - "Ascension" (drive to Ramona's house)
-Ginuwine - "Pony" (drive from Ramona's house)
-Eels - "Rags to Rags" (start of prep course)
-The Presidents of the USA - "Volcano" (kid's walkman)
-Chaka Khan - "Ain't Nobody" (Kevin's fantasy)
-Space - "Female of the Species" (Brittany's fantasy)
-Garbage - "Queer" (Jane's fantasy)
-The Cardigans - "Carnival" (Quinn's fantasy)
-Beck - "Where It's At" (Daria's fantasy)
-Stir - "Looking For" (arrive at Middleton)
-My Head - "Humbucker" (kid's walkman)
-Soundgarden - "Blow Up the Outside" (fraternity row)
-The Offspring - "All I Want" (Jake's old dorm)
-Butter 08 - "Butter of 69" (Heather's dorm)
-Alice in Chains - "Again" (kid's walkman)
-Gigantic - "Disenchanted" (Daria fixing papers)
-Big Daddy Kane - "Nuff Respect" (Quinn at frat party)
-Spice Girls - "Wannabe" (keg queen)
-The Bloodhound Gang - "Fire, Water, Burn" (Daria & Jane at Pizza King)
Middleton College was established as a colonial religious college in 1776. While not conclusive by any means, this does make for a strong argument in favor of Lawndale being located somewhere on the East Coast, since only the original thirteen British colonies existed at that time.
Last episode with the first version of the opening theme song ("You're Standing On My Neck"). The following episode ("Malled") uses the more standard second version.
The Wizard of Oz
Helen: I'll do the trick to you, you little twerp! And your [frat] brothers, too!
This sounds similiar to the Wicked Witch of the East, from The Wizard of Oz, when she says, "I'll get you, my pretty! And your little dog too!"