When the henchmen and Moliarty tunnel into the lab, the henchmen come out first and their noses are black but when one of them call to Moliarty to come on out, his nose is a pink/tan color then changes back to black after Moliarty comes up.
When Darkwing captures Moliarty's henchmen in the lab, it shows one of the henchmen wearing Moliarty's glasses even though Moliarty comes running in with his on.
When Professor Moliarty and his two henchmen break into the radioactive vault, one of the henchmen asks Moliarty why they have to wear the protective suits and one of the henchmen tells why in Moliarty's voice then is back to Moliarty in the next scene.
How could Darkwing blame his sixth arm for breaking the plate that fell on his right when that arm is on his left?
This episode aired out of order. Professor Moliarty's actual first appearance was in That Sinking Feeling
Gosalyn: I guess we better give Dad the antidote.
Launchpad: Uh, can't we wait one more day? I mean, those arms turned out to be pretty...uh...ahem, handy.
Darkwing: (Washing and drying dishes) Ooh, a fine pastime for a crime-fighter. Hmm, exploited by my own family.
Moliarty: Soon the surface dwellers will fear the might of Professor Moliarty, and the world will be my oyster.
Darkwing: Oysters are out of season, Moliarty.
Launchpad: Yeah, so maybe you'd just better clam up.
Lorenzo Music (The Spider) also did the voice of Garfield in the TV show Garfield and Friends.
First appearance of Professor Moliarty.
The entire episode is similar to a saga in the Spider-man series where Spider-man's mutation increases causing him to grow extra arms and become more Spider-like.
Professor Moliarity is a parody of Sherlock Holmes nemesis, Professor Moriarty
Darkwing: Oh! what a wicked web we weave, when first we practice to deceive!
Darkwing is slightly misquoting a passage from Sir Walter Scott's Marmion (1808):
"Oh! what a tangled web we weave
When first we practice to deceive!"
The episode title is a play on Arachnophobia which is the fear of spiders