In this episode, we see how Lance Boil looked before he was tiny, scrawny, and had a big red pimple for a head.
In this episode, we learn that Lab Rat's real name is Paul Squirfenherder.
Lili Francks, the voice of Insectiva, can be seen as one of the names on the cast list during the end credits, but Insectiva never appears in this episode.
When Ty and Abby are walking through the prison, the villains they pass by are Insectiva and Fartor.
Running Joke: Everyone calling Ty "Ladybug".
The characters' allergies:
Lab Rat: Mangos
The Director: Cats
Lance Boil: Fish
Although Lab Rat was afraid of germs and stink in this episode, but in the entire first season, Lab Rat absolutely hated bathing.
In the scene where Ty gives the greymold-filled Goop Shooter a trial run on a giant Venus flytrap (and killing it), one of the people cheering, oddly enough, is the younger version of Gary (Larry/Fartor's mean older brother) from Episode 3 ("Fartzilla")!
This episode's debuting guest villainess, Sarah Senia, was oddly never mentioned by name in the entire episode. (She is named, however, in the closing credits.)
Slim, the sewer worker from "The Slim Slime Man" makes a brief, wordless cameo appearance, now cleaning up trash around the amphitheater from the climax of that episode.
Kid Rot has developed an obsessive crush on Abby, whereas she has feelings only for Chester, not the monster he turned into.
While investigating in a swamp, Ty finds a leech and humorously taunts a horrified Abby with it. This was a reference to the previous episode, "Vein Drain," which explored Abby's fear of leeches.
When we see the Director trick-or-treating with his son, he's wearing a slime suit and his son is dressed up as Lance Boil.
Abby is scared of leeches.
This is the first episode in which Lance Boil's smoke-screen underpants are revealed.
First appearance of the episode's eponymous villain, Kid Rot.
This is one of the few episodes in which Abby and Ty must rely on pure ingenuity, without the use of their Slime-Suits or any other Grossology equipment, other than their communicators.
This is the first episode in which Lab Rat not only steps outside the Gag Lab, but also wears his blue Slime Suit.
First appearance of Hairball, Abby & Ty's pet kitten.
One of the names Ty uses to make fun of Abby's head lice condition is "Scabby Abby," which is a Grossology in-joke in itself; it was also the name of one of the "Grossologist" team experts of the popular Grossology traveling exhibition. Other members included "Snotty Scotty," "Marty Farty," "Bacteria Bart," and "Molly Mucus."