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Bijou calls the captain of the Pirate Team 'Captain Hamcook' in the game his name is Captain Hamstern.
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After The Rainbow Girls steal the Torch from Hamtaro and jump into the tree. If you have a really quick eye you will see that both Daisy and Ivy have green hair. (Daisy's hair is supposed to be yellow)
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At one point, the ham-hams all say "Hamcropolis" and Penelope's mouth moves as if she's saying it too.
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When Hamtaro tries lighting the ship's boiler with the everlasting torch, the torch goes out when Hamtaro stumbles backward. In the next scene, the torch is lit again.
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The ham-hams got the number of years, 8686, wrong several times. (They would say 3186 and 1386.)
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When the ham-hams are escaping from the Rainbow Girls on Junglebell Island, they all have a go on the pole jump. The ham-hams knock down the bar, but when the Rainbow Girls give it a go, the bar is back up again.
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When Boss removes his alien mask, he doesn't remove his hat first. When the mask comes off, the hat stays on his head.
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The commercial break is back in its normal position, but there's a screen flicker during the fade-to-black after the first portion of the episode.
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At least on the Cartoon Network version, there was a screen flicker during the switch from the first part to the second.
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During the birthday role-call at the end of the episode, Stan and Sandy are incorrectly listed as being born under the star sign Virgo. Their actual star sign is Gemini, as correctly stated on the official Hamtaro website.
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The screen flicker during the position the commercial break used to be that Cartoon Network seemed to have fixed is back once again.
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At the end, the Ham-Hams all say goodbye to Bijou. But if you listen really closely, you can hear Maxwell's voice with their's!
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According to Jingle, the couple knew each other for only a year but are already married.
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People in attendance at the wedding include: Laura and her parents, Kana and her parents, Mimi's parents, June, Kylie, Travis, Dr. Lion, and Goldie's son Zach.
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When Boss tells the story of how Bijou "fell" from the tree, he says that Bijou stepped onto the branch with her white foot. Like all hamsters, Bijou has a pink foot.
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When the Ham-Hams agree to start investigating, Penelope's mouth moves as though she's saying it with them.
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In this episode, Boss says "From sunflowers to chestnuts, we've got seeds for all your needs," when trying to peddle some seeds to a group of hamsters. But chestnuts aren't seeds.
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When Bijou knocks Boss over with the door, it swings into the clubhouse. In nearly all other episodes, the door swings outward, into the entrance tunnel.
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In one scene, when Laura is running from Travis, she starts going "bada bada," as if she's a hamster.
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In this episode, Claire is supposed to be Laura's best friend from her old neighborhood. But in "The Precious Letter," Melanie is her best friend.