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Evil Yumi has grey eyes and evil Ami has green eyes, unlike the real Ami and Yumi.
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The fat guy from Hungy Yumi apears in the episode Tooth Decay as a tooth fairy.
Although when Yumi in the episode Grindom Maidens said she doesn't wear any metal, she actualy was wearing her studded necklaces, wich is from metal.
Yumi took her studded bracelets off before going through the detector, but later when the security guard kicked her out she has the bracelets back on her.
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When Ami and Yumi are giving invitations to the alligators, the invitations in Yumi's left hand disappear.
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Yumi said they've been in 117 episodes and breaks the fourth wall with this statement, but it should be noted that this was only the 34th show, so the show isn't even up to that many episodes. Based on the three episodes (at seven minutes per program), this was the 102nd episode. The reference to "117" is that the show was to have been the 39th program made, hence that number. There are (as of this writing) five more shows that follow this.
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How could Kaz still be alive after Ami and Yumi became very old? If he's older than the girls, wasn't he suppose to be dead?
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The Sunikura Hydra has six heads instead of seven.
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This is the first time we see Ami and Yumi write Japanese characters.
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All of the other dialouges for the manga characters are written in Japanese, except for the "WAAA!!!" that Yumi made "Knoble Knight" say.
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When the delivery boy throws the donut into his mouth, the basket that was holding the newspaper disappeared and the reappeared in the following scene.
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"The HIt Parade", which is one of Puffy's albums, is listed as a song in the credits.
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At the beginning when Ami says, "Thank you, Yoyovia!", she says it in Yumi's voice.
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Goof: In "Rock Lobsters", when the mother lobster hits Ami and Yumi, Yumi is shown wearing Ami's boots.
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The case read "collapsing walls" but the walls were closing in on them, not collapsing.
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When Ami says "Tekirai" near the end of Fungus, the closed captioning says that she was speaking Japanese. But in previous episodes, when she said Tekirai, the CC said that she said Tekirai.
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When the fungus busts through Ami's drum, it tears it up. But in the next scene, it's fully repaired.
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In Run, Cat, Run, when Kaz was running in two of the scenes, the same background is seen moving across the screen instead of a different one.
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When Yumi is dreaming she says, "Thank you, Alpha Centauri!" She also says she had dreamt the band had sold out an entire planet. Alpha Centauri is a star, not a planet.
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The voice of Sunikura is not listed in the credits.
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In "Super Zero," Kaz was wearing his pajamas after he got up from his nap. In the next scene, where the inside of Sunikura's cave is, Kaz was wearing his shirt and pants.
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In "Ikkakujuu," when the barbarian guy comes in at the end to pick up his unicorn, he doesn't have a flower like Ami's on his headband in the first shot. But, in the second one, he does.