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In this episode Amanda tells Nick that she knew Nick was an Immortal (pre-Immortal) since the beginning. But in the earlier episode "The French Connection," when the doctor who tended to Nick's wound, falsely told Amanda that Nick had died, she wept and genuinely believed he had died from the injuries, but according to this episode, she should have been expecting him to revive anytime soon.
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Payton's use of a "holographic projector" edges this episode seriously into the realm of science fiction. It's also not quite clear why Amanda can't figure out which images of Payton are holograms, since they don't have any solid form and thus would be unable to parry a sword thrust or cut.
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Amanda acts as if Nick has never taken the head of an Immortal before. Arguably Nick deliberately took the head of an Immortal in the episode "Bloodlines" (deliberately shooting a pane of glass to decapitate his opponent), although this is the first episode where he does so quite so cold-bloodedly.
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Flashback: Bourgogne, France, 1565
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Flashback: Central Park, four years ago.
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Brennan heals far faster then is typically shown for Immortals: he immediately walks away from a falling death that took characters in previous shows and the original series several hours to "resurrect" from.
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Flashback: China Coast, 1907.
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Flashback: Theatre District, 1963
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Trivia: The Immortal sense of presence is given a name on-screen for the first time on either series: radar.
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Flashback: The Bahamas, 1720
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Flashback: Prague, August 20th 1968
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Flashback: New York, 1897
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Trivia: Amanda speaks Navajo.
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Trivia: Amanda has her first on-screen Quickening since her first original series episode, "Lady and the Tiger".
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When Detective Hoffman points her pistol at Amanda, we hear a hammer cock, but her weapon is clearly uncocked as she moves into view.