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When Gonard is making his "If space is time, then time is space," speech to Lily and Guano, a picture of a large black box and the earth appear in the background. This is most likely a reference to the Black Monolith from Stanley Kubrick's 2001: A Space Odyssey.
In this episode Mikey is pretending he is Ozu's son. But Guano was Ozu's son. It was revealed in the Christmas special.
Brozu has a Yes-Man just like Ozu, but his name is No-Man.
Throughout the episode, Ozu frequently mentions that he dislikes the excessive use of cards in anime plots. His business card even says "I hate cards."
When Mikey kisses Mitsuki, she deflates and blows away followed by hearts, and Mikey blushes leading some to believe that he may have feelings for her.
This is the first time Guano says something besides "Guano" in the LilyMu scene (he says "yaaaaay!")
The song chimney sweep Guano was whistling is "Chim Chim Cheree" from Disney's "Mary Poppins"
The items that were thrown through Ozu's old fence include a TV, a kitten, an old lady, and later, a bag of toys.
According to Guano, LilyMu is finished taping its first season since Mikey came to Japan.
In the flashback, young Ozu looks exactly the same as present day Ozu.
Mikey kisses Mitsuki in this episode.
What happened to everyone if Mikey did not win the scratch-off:
Ozu: Cancelled LilyMu after ratings plummeted.
Guano: Chimney sweeper with a bad cockney accent.
Gonard: Never stopped acting his LilyMu part.
Lily: Married Yoshi, got very fat, and has kids following her around.
Mitsuki: A washed up actress who refuses to accept reality.
Guano is Ozu's son.
Mikey told his parents that...
Guano = Bodyguard (Tallest in Japan)
Lily = Girlfriend
Gonard = Chef
Mitsuki = Mime
During the second LilyMu filming at the end of the episode, Mikey told Gonard that "It's time for la guitara!" which is Spanish for "the guitar."
The Lily Mu actors have several cell phones each.
When Gonard and Guano are giant, they're short enough to fit in the lab. But when both of them jump out, all the sudden they're bigger than nearly every building in Tokyo!
In the scene where Mikey and Gonard are destroying Dr. Igor's lab, watch closely. There's a scene where Mikey slices up Dr. Igor's bonsai tree. This is the same thing he did in Mikey Impossible to Ozu's bonsai tree, even with the exact same sword.
In keeping with the number 13 theme, this is also the thirteenth episode aired.
The numbers Gonard hits with his hands, 4 and 9, and when added equal 13, are an allusion to japanese speech, in which 4 is pronounced the same as death, while 9 is pronounced the same as suffering.