When Rupes Oberon went to free Optimatus, the villain is on a chess board chained to a chair, rather then on his spaceship, as he was last seen in "Acmegeddon (2)".
The String Theory is the theory that the entire universe is made of many different continually wiggling threads of energy, or strings. Some scientists theorize that the strings are straight, and some that the strings are circular. Keyboard Man's guitar and Zadavia's powers have absolutely nothing to do with the universe or it's strings, therefore, it's impossible for him to control it. In order for that to happen, he'd need to channel the energy of the strings, not Zadavia's.
When the Loonatics and Zadavia were back at Headquarters, Tech was in his civvies (military slang for civilian clothes) until the close up of Bootes Belinda. After the close up is done, you can see that Tech is in costume when he's backing away from the hologram.
We learned that Duck has a sister.
Danger Duck's lucky two-headed coin depicts his ancestor Daffy Duck dressed as Abraham Lincoln.
Lexi blasts a huge hole in the vat of nectar to destroy it, which can be seen continuously over several scenes. However, when Lexi and Duck work to create more nectar, the hole has mysteriously vanished.
There are numerous cameo appearances made by original Looney Tunes characters. Elmer J. Fudd was shown during Lexi's slideshow of the Fudd line. Also, the Stone-Age Fudd ancestor (though renamed here) was lifted straight from "Pre-Hysterical Hare" (McKimson, 1958). Granny, Sylvester, and Tweety were subjects in parodies of famous art work. Pepe Le Pew and Penelope were shown frozen as part of Otto's collection.
They recycled a clip of Ace flipping. The clip they recycled is the flip he did before turning around to push off the power core in "Loonatics On Ice (1-1, Production No. 101)".
If Electro J. Fudd is bald, then where did the hair come from? (Response: it was probably chest or armpit hair; also, we never actually see his full scalp, due to his electrode helmet, so we don't know if he's as bald as his ancestor was.)
Leix named a list, going backwards in time starting with Elmer J. Fudd, naming all the famous Fudd hunters.
Tech gave some animalistic features, like howling and whimpering.
How did Dr. Dare know where to find the Shield Of Medusa if he was petrified for over a year? (Response: He probably knew before he was petrified.)
Why didn't Tech use his magnetic powers on Stomper. His arms and legs are clearly made of metal.
The monster that the Loonatics battled is modeled exactly off of the monster Fuz-Zs that Dr. Zane unleashed in "Attack of the Fuzz Balls"
When Rev leaves his house to battle a monster in Acmetropolis, Rip sees him a second later on the tv news which is filming the Loonatics still fighting the monster. But in the next scene, Rev says that he didn't arrive in time to help the Loonatics fight.
The beginning of the episode recycles an audio clip from a past episode,"The Comet Cometh". The clip of Cookie talking to delivery boy Rev is the same one used by Mr. Bronski to address the pizza delivery boy.
There seems to be some inconsistency concerning Roadrunners' speed. In "Sypher", when Rev lost his powers and became a normal roadrunner, he was not very fast and could only talk at a normal speed. Rev's non-powered parents, however, are able to run and talk far faster than Rev when he had lost his powers.
When Rev and Tech are building the Robo Amigo at Rev's house, Tech falls off a cliff ala Wile E. Coyote.
First appearence of Sylth Vester and of The Royal Tweetums.
Duck's bedroom walls are covered in posters of himself.