Closed-captioning, at least on the version of the episode aired on Noggin, misspells Shelley's name as "Shelly." Additionally, Pansy's name is misspelled at one point as "Apsny" and a line of Bounce's in the second story is credited to Squirt, even though Squirt doesn't even appear in that story.
Stinky and his older sister Whiffy become Grace's parents in the end.
Dragon has a different voice in this season. But in "Bug-Versity", when Dragon sees Wiggle and Bounce running, he says "Hey. Wait for me" with his voice from the first season.
In "Bug-Versity," Pansy and Snowdrop briefly reprise the "La La Ladybugs" song from "Sing It Sister."
In "Bug Talk," Flint and Dazzle, the two fireflies, get married.
The Most Perfect Parent is available as Miss Spider's Sunny Patch Friends easy-reader book #11, written and illustrated by David Kirk.
Spiderus and Spindella's children are all named after poisonous plants: Mandrake, Belladonna and Poison Ivy (usually just called Ivy).
Wilver, who is introduced in this episode, is voiced by Juan Chioran. He provides the characters with a voice and style somewhat similar to Antonio Banderas (The Mask of Zorro, Shrek 2 and Shrek the Third.)
In "Night and Day" when Grace is lying in bed, she sings the "Hush, Little Ladybug" song that Stinky and Whiffy sang to her in the episode, "A Beetle-ful Family."
When Grace sees Stinky and Wiffy back from their trip, she calls them "Mom and Dad" for the first time.
Per the closing credits, Richard Clarkin provides the voice of Holley in this episode in place of Robert Smith. Clarkin's name appears in Smith's normal place in the credits. Additionally, Philip Williams (Ted), Jonathan Wilson (Ned), Barbara Budd (Betty Bettle), Hannah Endicott-Douglas (Grace) and Judy Marshak (Whiffy) are uncredited.
Shelley and Feather hold the first meeting of the Sunny Patch Strong Bug Club in the Ground House, which has not been seen since it was built in the first season story "Ground House Rules."
When Pansy is playing with Catty in her bedroom, she's humming the song that Spiderus and Snowdrop sung in "Lulla-Bug."
Miss Spider's pussy willow plushy is named "Catty." When Spiderus finds it and gives it to Ivy, she calls it "Daphne." When Pansy gives her a new one in exchange for it, she decides to call that one "Daphne" instead.
The closing credits include an end-tag in which Ned and Ted contemplate eating Stinky's awful cow-pies. This tag was not seen on Nick Jr.'s airing of this special, as they aired a promo over the credits.
"Pitch and itch" features the earliest appearance of Spiderus's daughter, Bella. Spiderus's children weren't born until "Lulla-Bug," suggesting this story was aired well out of production order.
"Fungus Among Us" is a rare Miss Spider story in which Wiggle can be seen without his cap on.
Berry, the crane fly who serves at the lifeguard at the Taddy Puddle, appears for the first time in "Ground Bound." He speaks with a bit of an Australian accent and uses several Australian phrases, including "wonky" (wobbly or loppsided), "mates" (friends) and "ripper" (fantastic). He also sometimes refers to the kids as little "bities," apparently in jest, as "bities" generally refers only to biting insects such as mosquitoes. Additionally, Bounce uses the phrase "good on ya'" (well done), when talking to Berry.
While Squirt and Wiggle say, "Bugs away" when they websurf, Petal says, "Bug out."
Both stories show someone getting hurt. In "Eight Legs Up," Snowdrop sprains a couple of her ankles and in "Spider Mom," Gus hurts his wrist.