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This is the second(and last) time in the series that you see Jax without his Bionic arms (referred to Bionics in the show).
The only appearance of Ruby.
First appearance of Ermac.
Last appearance of Kano.
The only appearance of Kabal.
Zara is not in the Mortal Kombat video games, she was created for the series.
When Rayden throws the orb into the portal and explodes, the Warrior King goes after it, but he is unnoticed by them. He travels to universe to universe (Street Fighter, Wing Commander, Savage Dragon) to recover the orb that came from his home world.
The only appearance of Quan Chi.
Final appearance of the Nomads.
First appearance of Shao Khan and noob Saibot.
First appearance of Kano.
The only appearance of the Lin Kuei master.
Last appearance of Cyrax.
Last appearance of Sektor.
The only appearance of Smoke.
The fate of Rain was undecided because he escaped.
First appearance of Motaro.
The only appearance of Rain.
This is the first time in the series that we see Jax without his Bionic arms (Bionics).
This is the first appearance of Sheeva.