This episode introduces two running gags that lasted throughout the series:
1) the complicated set of maneuvers required to open Peepers' locker
2) the water cooler that seemed to bubble and dispense paper cups with a mind of its own.
A sign in the school cafeteria says, "Masticate your food thoroughly."
This audition show was titled "Peepers". When the show was picked up by NBC, it was renamed to "Mister Peepers".
In the opening scene, the train station sign says, "Jefferson City, Altitude 1 foot 6 inches"