Starting in this episode (and continuing in the next three episodes that follow it,) various characters in My Gym Partner's a Monkey keep making jokes/ references to bloggers who complain about the show on the Internet.
Despite the school rule against eating other students, the opening credits show a fish being eaten by a bigger fish.
Seventh time that Jake Spidermonkey has appeared naked in an episode.
Even though it is against the rule to eat the other students at Charles Darwim Middle School, Adam Lyon is shown to have eaten David Coppertrout whole, just so that there wouldn't be a sequel to Animal School Musical.
At the beginning of Jake's ending song, Adam thinks Jake is talking about the friendship between the two of them. But when he says if it was possible or legal to marry, that should've hinted to Adam that Jake wasn't talking about him as gay marriage is possible and legal in several countries and in several states, with California being the most recent state to legalize it before the original airing date.
In the Understudy song, Lola Llama makes her second appearance, and she is using her neck extender as well.
This is the second instance of Jake making a face using his butt, the last time being in Yesterday's Funny Monkey.
Running Gags in this episode are: 1. Everybody breaking out into a musical number. 2.Jake trying to rhyme, and failing.
This is the first time that Horace has ever said anything.
The fourth appearance of David Coppertrout, the wish granting fish.
If in Flesh Fur Fantasy, Windsor became a new boy cheerleader, then why is Adam still shown as a cheerleader? Response: Windsor probably gave it up once he no longer needed to disguise himself as a human.
Adam Lyon: Jake, you missed your cue! Wait, why do I even care?!
Although Adam was completely uninterested in Ingrid's love song, when it seemed that Jake was singing about him he was very flattered.
In Ingrid's song, Adam mentions that he is lactose intolerant, which he last said in Yesterday's Funny Monkey.
When Windsor suggests that Jake sing about a hobby, he mentions his crystal figurine collection, last seen in Mandrill of the House.
In Lupe's song, she mentions the word, "Roshambo," which is another name for, "Rock-paper-scissors."
Adam's heart worms is a reference to the real species. Tapeworms are parasitic worms that can live anywhere in the human body, including the heart.
In the "Bug Stew" song, when Jake things of words that rhyme with "Doo-doo," he says "Free-mason," which is a reference to the fraternal organization of free-masons.
This episode is dedicated in memory of Robert Goulet.
Willy Wonka and the Chocolate Factory: When David Coppertrout starts changing Darwin Middle School from a regular animal school into a musical animal school, he fills it with lots of candy, including giant sized candy. This is reminiscent of the 1971 masterpiece starring Gene Wilder and Jack Albertson, having a building being filled with candy, including giant sized candy. Or it's 2005 remake Charlie and the Chocolate Factory starring Johnny Depp and Freddie Highmore.
Grease: When David Coppertrout changes the Spiffies from looking like nerds to looking like punk motorcyclists, the outfits the Spiffies wear are very similar to the ones worn by the Greasers in the 1978 movie musical masterpiece.
Foster's Home for Imaginary Friends : The mansion that pops Jake's float sort of looks like the Foster's Mansion.
Street Fighter II: the World Warrior : Some of the moves Adam Lyon and Jake Spidermonkey use while fighting each other are reminiscent of the moves used in the very popular 1991 video game.
Lupe's song mentions Hispanic forms of dance: The Mambo, popular in Cuba; the Tango, popular in Argentina and Uruguay; the Merengue, popular in the Dominican Republic; and the Rumba, also popular in Cuba.
David Coppertrout: Clap your heels together three times. This is the exact same line said in the 1939 version of The Wizard of Oz that Glinda says to Dorothy so that she can get home. Jake even wears the same ruby red slippers that Dorothy wore in the movie.
When You Wish Upon a Fish: This song is a parody of the song When You Wish Upon a Star in the 1940 Disney animated movie Pinnochio.
High School Musical: The title of Animal School Musical is a parody of the Disney Channel's live made-for-TV movie.