Yes it did. Frank(l)y, I'd be more inclined to believe that a character was not dead when there is any question of it, since Oda seems to be reluctant to kill off characters unless it is truly meaningful for them to die/have died. Case in point: Ace. He was sort of a mentor character, in a similar vein as ***SPOILER***
(from Gurren Lagan) and his death becomes a major impetus for Luffy to get serious-ish.
Edited on 04/05/2013 5:21pm
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Updated as of November 20, 2013. Spoilers are only for incidents that have not been shown in the anime yet.
Killed in the present:
1. Mr. 11 (shot in the head by the Billions) 2. Roshio the Executioner (killed by Bellamy the Hyena) 3. Edward "Whitebeard" Newgate (died a manly death) 4. Portgas D. Ace (killed by Akainu) 5. The guy judged by Enel when Nami was riding by on the waver
1. Gol D Roger (executed by the Marines) 2. Bellemere (killed by Arlong) 3. Kuina (sickness) 4. Monblanc Norland (executed) 5. Dr. Hiruluk (technically poisoned by Chopper, but committed suicide) 6. Tom (executed by the Marines with a DON!) 7. Jaguar D Saul (killed by Aokiji) 8. Brooke (died of an undisclosed illness, was revived by his Devil Fruit Powers) 9. Portgas D Rouge (died in childbirth) 10. Sabo (killed by Tenryuubito) 11. Fisher Tiger (killed by Marines) 12. Queen Otohime (killed by Hody Jones)