This is the first time Misty pulls Brock's ear to get him away from girls. This will now become a running gag.
This is the first time in a while Misty reminds Ash about her bike.
The Biker Gang is based on the Bikers from the games.
The background music that plays when Ash, Misty and Brock are happily riding across the bridge is the 'bike music' from the Game Boy games.
It is revealed that Jessie and James used to be part of the bike gang.
When Ash hands over the medicine to Nurse Joy he is only wearing one glove. After the medicine is taken from him he is wearing both gloves again.
Team Rocket: (after they pedal out of the water) This is a vicious cycle.
Chopper: Little Jim: the only member in the gang who had to use training wheels.
(After Ash and his friends hear the past exploits of Jessie and James when they used to be in the Bridge Bike Gang)
Ash: I kind of wish it still was a secret.
Pikachu: (monotone-like) Pika.
(Psyduck pokes itself on one of Cloyster's spines)
Brock: It doesn't look like Psyduck has a chance.
(Ash covers his mouth to hold back a fit of uncontrolable laughter)
Chopper: You're not scared of the storm are ya?
James: I'm not scared! I just wish I had my training wheels with me!
Chopper: From now on we're calling you Awesome Ash!
Tira: Mighty Misty!
Ash: Aw, gee, I'm not THAT awesome!
Brock: (to Tira) You can call me Honey!
(Tira slaps him)
Who's That Pokémon: Cloyster
Pokémon Debuts: Shellder, Cloyster, Golem