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The big weapon that Kambei and the group found belongs to Tanomo when he gets destroyed by the capital's cannon in the last episode.
Durian is a real fruit and it does smell quite noxious. However, it does not grow "anywhere" (it is a tropical rainforest plant). It also cannot be found in deserts and does not grow on Malaysian in orgin.
The Robotic Banits look like Bom-ombs from Wario World.
The Skio-Devito's look like Shy Guys, characters from Nintendo games.
While talking at the bridge, Kikuchiyo's sword is all red, but a few seconds later it returns to the normal red handle and gray blade.
In the last episode, Kirara wore her white priestess cloths. But in this episode, she is wearing her village cloths.
When Gorobei is about to kill one of the assassins, he has a cut on his forehead, but a few seconds later it's gone.
The scene where Kanbei has Katsushiro wait just inside the door and tells him to strike Gorobei as soon as he enters, is straight from the original film "The Seven Samurai."