When Fred and Daphne arrive at a temple screen, if look closely the skirt part of her dress, the top purple strip, has disappeared then re-appears.
(the gang rides the old handcar over to the dognappers' hideout...)
Shaggy: (mimicking train whistle) Whooo! Whoo-whoooo! Whoo-whoo!
Velma: We can get along without the sound effects, thank you.
Buck Masters: You blasted kids, why didn't you mind your own business?
Scooby: (as Shaggy reunites with Scooby--Shaggy's head is turned and Scooby has a witch doctor's mask on his face...) Ri, Raggy.
Shaggy: Ri, Rooby.
The ghost of Geronimo appears in Scooby Doo Night of 100 Frights.
Villian: Buck Masters
Reason: He was kidnapping competitors' dogs so he could win the dog show.
This is the only episode in the original series that features the gang taking a normal, non-supernatural mystery. Although there does turn out to be some mysterious, supernatural phenomenon, we don't see or hear about until halfway through the episode.
Dignity, Velma! As she and Shaggy are flailing to avoid the attack of bats, Velma is kicking her knees up high enough to show her panties (they were colored the same as her skirt).
This is the fist time Shaggy eats a Scooby Snack.
This is the first time Daphne is tied up.
If you look closely on the passenger train, you can see parts of the Santa Fe logo on the locomotive.
This episode features one of the rare instances of a genuinely affectionate relationship among the characters. Fred (of all people) states that "Scooby-Doo is a dog, and we love him- very much."
This is the only episode which shows the inside of the Mystery Machine with a high-tech setup instead of a typical van.
One of the few episodes in this series to include a daytime scene. The New Scooby-Doo Movies had lots of daytime scenes.
First time Velma says: "My glasses; I can't see without them!"