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Environmental pollution is a major theme of this episode. It is one of the few episodes that makes note of the fact that (despite being a children's show) Mr. Jamerson is smoking a cigar.
This episode is available on the VHS Marvel Comics Video Library Volume 1 "The Amazing Spider-Man: The Origin Of Spider-Man".
It is said that the Spidey toys are an inch tall. AFTER Spidey reveals that HE is a half-inch, he is still able to hide with the toys--even though he is half as short.
Near the end of the episode Kraven reaches for his belt to access some weapons contained in it. Ka-Zar then proceeds to pull the belt off of Kraven, yet in the next scene Kraven is again seen wearing the belt.
Norman Osborn should not have been able to survive a severe train derailment and explosion like he did here. Apparently, the other passengers and crew members all died in the wreck.
When Jameson and Aunt May are on the phone talking to each other, and Aunt May tells him that he should get someone else because Peter is studying, it is loosely based on the Spider-Man (1967) episode "Horn of the Rhino" which itself was based on The Amazing Spider-Man #41.
When Dr. Octopus is underwater stealing the world's oil supply it is hard to tell the difference from his mental arms and the pipes stealing the oil.