In the scene where Morbius attacks Debra Whitman, right before the Man-Spider gets there, Morbius's hair changes from blue to black back and forth 3 times.
Kraven: (About Man-Spider) Do not hurt him!
Punisher: I'm not gonna hurt him; I'm gonna disintergrate him. Won't be any nerve endings left to feel the pain!
Punisher: I looked the devil square in the eyes... and I blinked.
Kraven: 'A man that studieth revenge keeps his own wounds green.' Francis Bacon. Revenge is a foolish reason to hunt! Punisher: Maybe, but it works for me.
Kraven: Well, my little Calypso, you have done it again! Spider-Man: Calypso? Dr. Crawford: In Mythology, Calypso was a Goddess, who offered the gift of life to Odysseus. Spider-Man: Well, I guess you're my Calypso too.
This is the first animated appearance of Man-Spider, from Marvel Fanfare #2. In the comic however, Peter did not change into this because of a mutation disease. Instead, he was zapped by a device created by the Brainchild that made him go through a de-evolutionary change. Peter was turned back to normal after Carl Lykos (Sauron) absorbed his now altered energy.
In comics, Kraven's last name was spelled "Kravinov," though here, it's spelled "Kravenoff."
In the unedited version of this episode, Kraven says to the Punisher "It smelt the gun powder from that bomb explosion years ago!" The "explosion" was the reference to when the World Trade Center was bombed in 1993, eight years before it met its ultimate fate. All references to the World Trade Center have been edited out in ABC Family rerun episodes. Man-spider was hiding in the WTC parking garage.
"It is written that a man may build himself a throne of bayonets, but cannot sit on it."
Kraven's line is an approximation of a famous Boris Yeltzin quote usually translated as "You can build a throne out of bayonets, but you can't sit on it for very long."