The ship during this episode has to journey through electric seaweed. Which is a nod to a level on the original TMNT video game on the NES.
Robbie Rist (voice of Mondo Gecko) played the voice of Michelangelo in the original 1990's live action TMNT trilogy.
The campfire scene in the woods is similar to the scene where Splinter talks to the turtles in the 1990 Teenage Mutant Ninja Turtles motion picture.
When Baxter interrupts the Turtles from curing April's dad, he demands they give him the retromutagen. This is a goof because Baxter shouldn't know what the Turtles were doing, and was only interested in revenge at the start of his attack.
Raphael claims that he "doesn't do rings." This is kind of ironic considering that his voice actor, Sean Astin, portrayed Sam in the Lord of the Rings films.
Leonardo's cape has a rubber duck wearing a shower cap on it.
In the first half, Stockman has the mutagen collar around his neck, but in the second half when Splinter is brought to the Shredder, the mutagen collar on Stockman's neck is gone.
Among the mutants imprisoned by the Kraang are Snakeweed, Spyder Bytez, Dr. Rockwell, Pigeon Pete, a Parasitic Wasp, Mr. O'Neil, a Squirrelanoid, and Slash.
The characters' worst fears are:
Leonardo: Failing as a leader
Donatello: April hating him
Michelangelo: The Squirrelanoids
The girl with purple hair and wears glasses is Irma, based on the character from the first cartoon series.
One part on the intro where Michelangelo throws a stink bomb at a foot ninja is taken out of the intro due to how the stink bomb was never use in the first season.
The intro of the show has three changes:
1. The scene with the Foot Clan is replace with the villains who are Shredder, Karai, Dogpound, Fishface, Snakeweed, Spider Bytez, Newtralizer, the Rat King, Mrs. Campbell, and Krang Prime. For some unknown reason, Baxter Stockman and Spyroach are not in the intro.
2. Metalhead walking by where Donatello is working on the Shellraiser.
3. A skeleton is seen at a wall when the ninja turtles are jumping on the roof.
In this episode, we learn that the Pulverizer's real name is Timothy.
In the 2003 series, Leatherhead is friends with the Utroms. While in this series, Leatherhead is enemies with the Kraang.
The character Murakami is voiced by Sab Shimono, who portrayed the villain Lord Norinaga in Teenage Mutant Ninja Turtles III.