Goof: Ike is seen in the chorus after Cindy tells them to leave, then he is seen at the Candy Bar, about to turn on the jukebox, before Carl stops him.
Nathan, Keith Alcorn's son, had no lines in this episode.. But he was credited by Candi Milo in the credits.
Both Candi Milo and Jeff Garcia were credited with doing the voice of Nathan.
Ms. Fowl (singing): Oh, A is a letter that we all should know. Without it, we couldn't spell "ant!" B is a letter that we all love so. Without it, there'd be no BOTULISM! (gets attacked by mutated Twonky)
Jimmy: Sheen, how do you like your singing voice to be beloved by everyone in town?
Sheen: I don't know... I have to... Okay!
Jimmy: This isn't over, people! If we don't blast that monster back to the comet in the next ten minutes, he's stuck here for another year!
Sam: That's bad.
Sheen: I'll just sing to him everytime he wakes up.
Miss Fowl: That's worse!
Sheen: (singing) Home, home on the range! Where the frog and the wolverine dance! And the cowboys say nope to deodorant soap! And the--
Libby: Shall I do the honors?
Carl: Be my guest.
Libby: RUN FOR YOUR LIVES!!!!
Cindy: (as Jimmy's figuring out why the twonkies mutate; sweetly) Um, Jimmy? If you're done talking to yourself ... (yelling) we have a situation here!!
"Die Again Tomorow Forever" (the movie from "Rescue Jet Fusion") is playing in the theater scene.
In the movie theater you can see Eustace Strytch from Billion Dollar Boy
The last stage of the twonkie is not called a Gromp. The Gromp stage of the twonkie was never shown in the series but was shown in the Jimmy Neutron game in which the Gromp wasn't even close to the size of the twonkie's final stage.
The three stages of twonkies are Twonky (normal), Stomper (enraged), and Final (conglomeration).
This special is a spoof of Gremlins and Critters.
First appearance of Graystar, which is an actual band.
This special can be seen on the DVD Attack of the Twonkies.
Sheen: (singing) Totally singing, totally singing, totally singing, right in your face! You smell like a monkey, a disgusting monkey, you're totally funky, go take a bath!
This is to the tune of "Flight of the Valkyries."
Sheen: (singing) UltraLord! UltraLord! Fighting crime with his ultrasword! He can fly, what a guy! Watch the criminals wave bye-bye! Who's got electric booties? Loves his crime-fighting duties? And is immune to cooties? His name is Ul-tra-Looooooord!
This song is to the tune of the theme from Spider-Man.
Sheen: (singing) Oh, I'm singing to a monster in mid-air ... I'll be singin' to a monster in mid-air! Oh, I'm feeling so dejected, from that chorus I'm rejected, but I'm singing to a monster in mid-air!
Sheen's song is to the tune of "She'll Be Comin' 'Round the Mountain."