Rance Howard (father of Ronnie & Clint) makes his first appearance, in this episode as a bus driver
When Barney pulls off the wall of the cell, you nearly immediately see him in the window. However, this can't be, since the window is about 8 feet off the ground, and the car that he pulled it off with couldn't be under the window. Also, you can see the whole cell move indicating that it is not solid.
At the dinner scene cousin Virgil knocks a platter of roast onto Andy's lap, Andy was wearing his sherrifs uniform. At the end of the evening as Andy is seeing Barny and Virgil out the door he is still wearing his uniform... however it is spotless.
Although Floyd laments numerous times about having a "Two Chair Shop" this is the one and only time that Floyd's ever sports two barber's chairs
This is one of only three times that a bathroom is actually shown in the series. And in both of those other episodes (Gomer the House Guest and Howard's Main Event), as in this one, only the sink is shown. Interestingly, based on the layout, apparently the Jail/Courthouse has no bathrooms at all and the cells have no toilets - although one cell does have a sink!
This was Frances Bavier's favorite episode.
Julie Adams (County Nurse Mary Simpson) was well-known at the time for playing Kay Lawrence - the abducted girl in the Creature From the Black Lagoon (1954).
Andy is shown with a cast on his arm (supposedly from a sprained wrist he got bringing in the Gordon boys). However, the cast has little or no impact on the plot and Andy is still wearing it in the next episode. This is because, in real life, Andy Griffith actually sprained his wrist driving his fist through a wall. The reason for this seemingly uncharacteristic outburst has been debated for years. Some say it was because of a fight he had with his then wife Barbara (who appears in The Song Festers), but no one really knows for sure.
Children of all ages might recognize Bert Miller's unique, high, raspy voice. Sterling Holloway, who played Bert in this episode, is most famous for being the voice of Disney's beloved Winnie the Pooh!
Ellen Brown has the distinction of being one of very few women to ever be seen inside Floyd's Barbershop.
When Barney tries to extract information from Otis, it is revealed that his house number is 411. 411 is the telephone number for information.
During the scene in which Barney attempts to use hypnosis to find out where Otis is getting his liquor, it is revealed that Barney's address is 411 Elm Street. Coincidentally this is the exact same address as the Texas School Book Depository in Dallas where Lee Harvey Oswald shot President Kennedy two years later.
Aunt Bee mentions she moved to Peoria when she was 18 and was "so skinny".
Karen must be from the opposite side of the family than Mary Grace, Thelma Lou's "other" cousin - who Barney "affectionately" calls a dog!
In this episode, Andy says that the combination to the Mayberry Bank's vault was lost and that a back door was cut into it to allow access. But, in Season Three's The Bank Job, Barney gets locked in the vault and has to bust through the bank's back wall to get out.
Frank mentions a trinket he got from the Saint Louis World's Fair. This international exposition, which was held in St. Louis, Missouri from April 30 until December 1, 1904, celebrated the centennial of the Louisiana Purchase and was officially called The Louisiana Purchase Exposition.
Aunt Bee wears glasses in this episode for what I believe is the first time.