"The Secret Room" is the 2nd episode to have "secret" in the title.
Secret Code - Destination Sun - 3571
Secret Code - The Secret Room - 1739
Anita's communicator ring tone is the same tune as the show's theme song.
Professor Professor's Invention(s): Hologram Backpacks.
The Fluffy Bunny Show: In "Destination Sun," the agents enter the show with a submarine, and in "The Secret Room," the agents use a car jack to lift up the screen that's airing The Fluffy Bunny Show.
Changed Daily's Names: Oinky Doinky and Patty Cake.
(Doctor Doctor keeps handing fake controllers to Professor Professor, then sees Anita)
Doctor Doctor:(Hands her the real controller) Oh, can you hold this for me, please?
(Anita takes it)
Doctor Doctor: And this one, and this one, and this-- wait! THAT'S THE REAL CONTROLLER!
This episode aired as part of Nicktoons Network's "March Mayhem."
In The Secret Room, the line, "We don't need the moon and stars, we have each other." is most likely a reference to the like, "Let's not ask for the moon...we have the stars," from the movie Now, Voyager.