The ant armies wear trench coats and fedora hats similar to those worn by Carmen Sandiego. Where in the World is Carmen Sandiego? was also airing on Fox Kids at the time this episode premiered.
Therapist: Just relax. I am a professional.
The Tick: You're just some head in a water cooler!
Fishboy: Where's The Tick? You told my mom The Tick would be here.
Die Fledermaus: Listen Fishboy, I told your mom a lot of things.
The Tick: My mind has always been my Achilles' heel!
The Tick: Isn't sanity really just a one trick pony, anyway? I mean, all you get is one trick, rational thinking! But when you're good and crazy… ooh hoo hoo hoo… the sky's the limit!
Die Fledermaus: Let me handle this.
American Maid: You're going to handle this?
Die Fledermaus: Of course, they're ant women, right? ...Hey Honey, I'm a Scorpio. I like long walks on the beach, good conversation and spelunking, how 'bout you?
This episode marks the 2nd appearance of Fishboy, lost prince of Atlantis. He was first seen in season 1's "The Tick vs The Tick".
The Tick riding the falling ant and cheering is a nod to the famous scene of a man riding on a falling bomb from the film Dr. Strangelove. The recurring mad scientist character, who appeared in this episode, is also a parody of the titular character from the film.
Taft is similar to the 70's version of Shaft, even down to the theme song.