When Faragonda gives the test to see who will go into the Underrealm, the camera closes in and we see Musa between Flora and Tecna. When it backs away, Musa is now between Flora and Layla.
After Bloom, Stella, and Layla defeat the monsters, Layla is about to lose her Winx and go back to normal mode. There's a part where she kneels down because she's tired, and look closely, Layla is wearing her mission boots instead of her Winx boots!
Stella:Oh no! Sky grab the royal make up case!
(Sky misses it completely and it falls off the Clift)
Sky: Oh I'm sorry Stella. I'll get you a new one
Stella: You can't! It's Couture!
Layla: Now slide to the back seat.
Layla: Come on, how am I supposed to drive with you sitting there?
Layla: So what is it that you heroes do, exactly?
Stella: Why don't you tell her, Brandon?
Brandon: Well, we're not officially heroes until we graduate, but we do all sorts of heroic activities, like rescue operations. We also battle monsters and evil forces in general.
Layla (talking to Tecna): You're right why would we need high school boys?
Brandon: We're not just high school boys, we're heroes. And what does that mean?
Sky: (points with both hands) We go to Red Fountain.
Musa: My dad said once, "It's cool to try and fail, but to not try is not cool."
Timmy (after increased the ships speed to almost maximum): Isn't it great?
Layla: It's really impressive, but it'd be more impressive, if you were going in the right direction
Brandon: Turn around, Timmy!
When Layla goes to the Underrealm, Piff says "Bye, Layla!", but it's stated that her first word is "cocoa" in the Season 2 finale.
In Rainbow version Layla says that she sealed the door with magic glue, and in later episodes it is revealed that her power is the Morfix, a pink fluid that she can bend to her will.
This episode is also known as (English title): Rescue Mission.
Plot Alternation: In the European version, Bloom sees Layla transform for the first time in the UnderRealm .
Original Plot: Faragonda told Layla to go find the Pixies, but she chose Bloom and Stella to go on the mission because their powers were the strongest. She summoned the scenery of Shadowhaunt to show Musa and Flora that their powers weren't strong enough. (She told Tecna that she would be tracking the mission at Alfea.)
Lalya uses her Winx power to control water molecules for the first time.
This is the first time we've seen Layla use her Winx to transform.
The Italian episode is titled Rescue Mission. Translation: is the same.