At about 7:30 in the episode, Frasier has just announced to Martin and Daphne that "Freud is the poor man's Jung". As Niles is responding, "I never thought I'd live to see the day -- perhaps this is why I was spared", watch John Mahoney. It seems he's confused as to whether or not they're filming. He seems to check with someone in front of the stage, then looks up high behind himself as if to check a monitor. Finally, he looks straight at the camera.